Esterase Activity of Streptolysin-O
نویسندگان
چکیده
منابع مشابه
Cytochemical electron microscopic localization o esterase activity in Lactobacillus casei.
Enzymes capable of hydrolyzing thiolacetic acid in two strains of Lactobacillus casei were localized by the formation of electron-opaque PbS deposits in vivo in the presence of Pb(NO(3))(2). By electron microscopy, the deposition of PbS appeared primarily at the plasma membrane and at the outer surface of the cell wall.
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The definition of rheumatic fever has been broadened of recent years and many people take it to include not only articular and skin manifestations but also all those signs that are commonly regarded as evidence of an active carditis. While this conception may be pathologically correct and at times convenient in use, it is clear that there are many cases of rheumatic fever where there is no proo...
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Pore-forming toxins are utilized by bacterial and mammalian cells to exert pathogenic effects and induce cell lysis. In addition to rapid plasma membrane repair, macrophages respond to pore-forming toxins through activation of the NLRP3 inflammasome, leading to IL-1β secretion and pyroptosis. The structural determinants of pore-forming toxins required for NLRP3 activation remain unknown. Here, ...
متن کاملIsolation and identification of two hemolytic forms of streptolysin-O.
Streptolysin-O was isolated from culture supernatants of group-A beta-hemolytic streptococci (Richards strain) by ammonium sulfate and polyethylene glycol precipitation, DEAE-ion exchange chromatography, preparative isoelectric focusing, and chromatography on Sephacryl S-300. Two forms of the toxin possessing similar hemolytic capacity were identified. The native toxin was a single polypeptide ...
متن کاملHeterogeneity of hemolytic efficiency and isoelectric point of streptolysin O.
Using thin-layer agarose gel isoelectric focusing overlaid with thin-layer erythrocyte agar plates, we found that crude streptolysin O (SLO) consisted of a variety of hemolytic components with different isoelectric points (pIs) and that the distribution of pIs in crude SLO was different even in samples which were produced from a single strain of Streptococcus pyogenes under similar conditions. ...
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ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1972
ISSN: 0022-1287
DOI: 10.1099/00221287-73-2-387